Difference between revisions of "Part:BBa K2924035"
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<partinfo>BBa_K2924035 short</partinfo> | <partinfo>BBa_K2924035 short</partinfo> | ||
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Fluorescent protein mVenus, codon optimized for <i>Synechocystis sp. </i> PCC 6803 | Fluorescent protein mVenus, codon optimized for <i>Synechocystis sp. </i> PCC 6803 | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | mVenus is a yellow fluorescent protein derived from gfp and its derivative eYFP, which stem from Aequorea victoria. Its excitation wavelength is at 515 nm and it emits light of 527 nm wavelength. The protein has a size of 26.9 kDa and is usually found as a monomer. | + | mVenus is a yellow fluorescent protein derived from gfp and its derivative eYFP, which stem from <i>Aequorea victoria</i>. Its peak excitation wavelength is at 515 nm and it emits light of 527 nm peak wavelength<sup>1</sup>. The protein has a size of 26.9 kDa and is usually found as a monomer. |
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| + | This part was characterized with the P<sub>cpc560</sub> Promoter in terms of fluorescence, protein product and used to test a CRIPRi-dCas9 gene knock-down in the composite part [https://parts.igem.org/part:BBa_K2924036 BBa_K2924036]. | ||
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<partinfo>BBa_K2924035 parameters</partinfo> | <partinfo>BBa_K2924035 parameters</partinfo> | ||
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| + | ===References=== | ||
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| + | 1: Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580. | ||
Latest revision as of 16:05, 21 October 2019
mVenus
Fluorescent protein mVenus, codon optimized for Synechocystis sp. PCC 6803
Usage and Biology
mVenus is a yellow fluorescent protein derived from gfp and its derivative eYFP, which stem from Aequorea victoria. Its peak excitation wavelength is at 515 nm and it emits light of 527 nm peak wavelength1. The protein has a size of 26.9 kDa and is usually found as a monomer.
This part was characterized with the Pcpc560 Promoter in terms of fluorescence, protein product and used to test a CRIPRi-dCas9 gene knock-down in the composite part BBa_K2924036.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 630
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]
References
1: Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.