Difference between revisions of "Part:BBa K3102022:Design"

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We mutated a Cytosine into Guanine at the 1609bp position (C1609T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.
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We mutated a Cytosine into Guanine at the 1566bp position (C1566G) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.
  
 
===Source===
 
===Source===

Latest revision as of 10:22, 16 October 2019


ACS (Acetyl-coenzyme A synthetase) Shewanella Oneidensis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 931
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Cytosine into Guanine at the 1566bp position (C1566G) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.

Source

This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-2522#tab=TU

References

Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.

Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.