Difference between revisions of "Part:BBa K3102031:Design"
(→Design Notes) |
(→Design Notes) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. | + | We mutated the ACS sequence with a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (BBa_K3102021) |
===Source=== | ===Source=== | ||
Latest revision as of 10:41, 16 October 2019
T7-RBS-ACS-Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 408
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 837
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated the ACS sequence with a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (BBa_K3102021)
Source
E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11448
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.
Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.