Difference between revisions of "Part:BBa K2938007:Design"
 
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===References===
 
===References===
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[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.
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[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.
 +
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[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.

Latest revision as of 09:37, 12 October 2019


Cyt1Aa With Strep Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 96
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Strep-tag fused to C- terminal


Source

Gibson assembly. Tag was added through primers. Cyt1Aa was isolated from pVE4-ADRC plasmid by PCR. and was optimized for E.Coli

References

[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.

[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.

[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.