Difference between revisions of "Part:BBa K3102027:Design"

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===Source===
 
===Source===
  
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None
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E.coli, sequence found through Trypanocyc
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http://vm-trypanocyc.toulouse.inra.fr/ECOLI/sequence?type=GENE&object=EG11961
 +
 
 +
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
  
 
===References===
 
===References===

Latest revision as of 09:44, 12 October 2019


T7-RBS-LLDP-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 460
    Illegal AgeI site found at 923
    Illegal AgeI site found at 1361
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 1532
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

E.coli, sequence found through Trypanocyc http://vm-trypanocyc.toulouse.inra.fr/ECOLI/sequence?type=GENE&object=EG11961

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8.

Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9.