Difference between revisions of "Part:BBa K3102024"

Latest revision as of 14:53, 14 October 2019

T7-RBS-EG1-ter (secreted EG1)

This T7-RBS-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesizing EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102041) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1161
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 104
Illegal AgeI site found at 362
Illegal AgeI site found at 452
Illegal AgeI site found at 1289
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3102024 short</partinfo>
-This T7-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesize the EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102041) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.
+This T7-RBS-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesizing EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102041) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.
-This constructs is enriched with primer, T7 promoters, RBS and terminator.
+This construct is enriched with  T7 promoter, RBS and terminator.