Difference between revisions of "Part:BBa K3238000"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3238000 short</partinfo> | <partinfo>BBa_K3238000 short</partinfo> | ||
| − | This part is the | + | This part is used to repress the ompW BBa_ under anaerobic conditions. |
| − | + | Transcription of the pdh operon is controlled by two major promoters: the upstream promoter Ppdh generates a pdhR-lpdA readthrough transcript, and the internal promoter Plpd generates independent lpdA transcript. The primary pdh promoter Ppdh is negatively autoregulated by PdhR, the product of the first gene in this pdh operon | |
| − | + | <h1>Biology</h1> | |
| − | < | + | <p> This system is composed by the response regulator ArcA and the sense kinase ArcB. When E. coli is under Anaerobic conditions ArcB has kinase activity, so it phosphorylate ArcA protein which is activated and repress the gene related with aerobic metabolism.</p> |
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Latest revision as of 03:59, 22 October 2019
Pyruvate dehydrogenase complex promoter, pyruvate inducible
This part is used to repress the ompW BBa_ under anaerobic conditions. Transcription of the pdh operon is controlled by two major promoters: the upstream promoter Ppdh generates a pdhR-lpdA readthrough transcript, and the internal promoter Plpd generates independent lpdA transcript. The primary pdh promoter Ppdh is negatively autoregulated by PdhR, the product of the first gene in this pdh operon
Biology
This system is composed by the response regulator ArcA and the sense kinase ArcB. When E. coli is under Anaerobic conditions ArcB has kinase activity, so it phosphorylate ArcA protein which is activated and repress the gene related with aerobic metabolism.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]