Difference between revisions of "Part:BBa K3282000"
 
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This part contains coding sequence of Escherichia coli’s K12 (substr. MG1655) arsenical resistance operon repressor (arsR, Gene ID: 948013, Uniprot: P37309) codon-optimized for Escherichia coli Nissle 1917. The arsR gene which encodes for regulatory proteins, is a part of the ars operon of the E. coli conjugal plasmid R773 (1). ArsR is a 117 residue protein, that has been shown to be a trans‐acting repressor and is a sensor of environmental As(III). This 13 kDa protein belongs to the ArsR family of metalloregulatory proteins that respond to a variety of metals including As(III), Sb(III), Cd(II), and Zn(II) (2).
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This part contains coding sequence of ''Escherichia coli'' K12 (substr. MG1655) arsenical resistance operon repressor (''arsR'', Gene ID: 948013, Uniprot: P37309) codon-optimized for ''Escherichia coli'' Nissle 1917. The ''arsR'' gene which encodes for regulatory proteins, is a part of the ''ars'' operon of the ''E. coli'' conjugal plasmid R773 [1]. ''arsR'' is a 117 residue protein, that has been shown to be a trans‐acting repressor and is a sensor of environmental As(III). This 13 kDa protein belongs to the ''arsR'' family of metalloregulatory proteins that respond to a variety of metals including As(III), Sb(III), Cd(II), and Zn(II) [2].
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The part was evaluated in the composite parts [https://parts.igem.org/Part:BBa_K3282003 BBa_K3282003] and [https://parts.igem.org/Part:BBa_K3282004 BBa_K3282004] expressed in pUC19 backbone. The composites have the same RBS ([https://parts.igem.org/Part:BBa_J61109 BBa_J61109]), terminator ([https://parts.igem.org/Part:BBa_B0012 BBa_B0012]) with two different promoters T7 ([https://parts.igem.org/Part:BBa_I719005 BBa_I719005]) and Tac ([https://parts.igem.org/Part:BBa_K3282006 BBa_K3282006]). The four composite parts were tested to determine their Arsenic uptake capability.
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References
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1. Carlin, A., Shi, W., Dey, S., Rosen, B. (1995). The ars operon of Escherichia coli confers arsenical and antimonial resistance. Journal of Bacteriology, 177 (4), 981-986; doi: 10.1128/jb.177.4.981-986.
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2. Rastorguev, S.M., Zavilgelsky, G.B., Tchurikov, N.A. (1998). IncI1 plasmid R64 encodes the ArsR protein that alleviates type I restriction. FEBS Lett. 426, 21–23.

Latest revision as of 16:39, 17 October 2019

This part contains coding sequence of Escherichia coli K12 (substr. MG1655) arsenical resistance operon repressor (arsR, Gene ID: 948013, Uniprot: P37309) codon-optimized for Escherichia coli Nissle 1917. The arsR gene which encodes for regulatory proteins, is a part of the ars operon of the E. coli conjugal plasmid R773 [1]. arsR is a 117 residue protein, that has been shown to be a trans‐acting repressor and is a sensor of environmental As(III). This 13 kDa protein belongs to the arsR family of metalloregulatory proteins that respond to a variety of metals including As(III), Sb(III), Cd(II), and Zn(II) [2].


The part was evaluated in the composite parts BBa_K3282003 and BBa_K3282004 expressed in pUC19 backbone. The composites have the same RBS (BBa_J61109), terminator (BBa_B0012) with two different promoters T7 (BBa_I719005) and Tac (BBa_K3282006). The four composite parts were tested to determine their Arsenic uptake capability.


References

1. Carlin, A., Shi, W., Dey, S., Rosen, B. (1995). The ars operon of Escherichia coli confers arsenical and antimonial resistance. Journal of Bacteriology, 177 (4), 981-986; doi: 10.1128/jb.177.4.981-986.

2. Rastorguev, S.M., Zavilgelsky, G.B., Tchurikov, N.A. (1998). IncI1 plasmid R64 encodes the ArsR protein that alleviates type I restriction. FEBS Lett. 426, 21–23.