Difference between revisions of "Part:BBa K3088002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphtheria toxin detection with observable green fluorescent protein. | This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphtheria toxin detection with observable green fluorescent protein. | ||
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| + | This part was successfully sequenced and the sequencing showed the correct result. | ||
Latest revision as of 07:03, 20 October 2019
HBEGF/ENVZ Chimeric
Heparin-binding EGF-like growth factor (HB-EGF) is a unique receptor of the diphtheria toxin. EnvZ, on the other hand, is a member of two-component regulatory system that is important for bacteria osmoregulation. When diphteria toxin binds to HB-EGF, it will phosphorylate EnvZ and activates the signalling cascade pathway. Once EnvZ is phosphorylated, OmpR (a transcription factor of OmpC promoter) will also be phosphorylated. The phosphorylated OmpR will initiate OmpC transcription and express the gene following OmpC promoter.
Usage and Biology
This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphtheria toxin detection with observable green fluorescent protein.
This part was successfully sequenced and the sequencing showed the correct result.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]