Difference between revisions of "Part:BBa K2983000"

 
 
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<partinfo>BBa_K2983000 short</partinfo>
 
<partinfo>BBa_K2983000 short</partinfo>
  
This part is the Zeta-Up sequence which together with the Zeta-Down (BBa_K2983001) allow random integrations in Yarrowia lipolytica Po1d strain JMY195 [1] or at a zeta docking platform in Po1d derivative strains like JMY2033 [2] which has the zeta platform at the ura3-302 locus or JMY1212 [3] which has the zeta platform at the leu2-270 locus
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This part is a Zeta sequence.
  
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===Usage and Biology===
 
===Usage and Biology===
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Together with the Zeta Down ([[Part:BBa_K2983001|BBa_K2983001]]), this part allows random integrations in ''Yarrowia lipolytica'' Po1d strain JMY195 [1] or at a zeta docking platform in Po1d derivative strains like JMY2033 [2] which has the zeta platform at the ura3-302 locus or JMY1212 [3] which has the zeta platform at the leu2-270 locus.
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===References===
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[1] Barth G, Gaillardin C. ''Yarrowia lipolytica''. In: Wolf K (ed) Non conventional yeasts in biotechnology. Springer, Berlin (1996) 1, 314-388.
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[2] Lazar Z, Rossignol T, Verbeke J, Crutz-Le Coq AM, Nicaud JM, Robak M. Optimized invertase expression and secretion cassette for improving ''Yarrowia lipolytica'' growth on sucrose for industrial applications. J Ind Microbiol Biotechnol (2013) 40, 1273-83.
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[3] Bordes F, Fudalej F, Dossat V, Nicaud JM, Marty A. A new recombinant protein expression system for high-throughput screening in the yeast ''Yarrowia lipolytica''. J Microbiol Methods (2007) 70, 493-502.
  
 
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Latest revision as of 22:25, 21 October 2019


Zeta Up

This part is a Zeta sequence.

Usage and Biology

Together with the Zeta Down (BBa_K2983001), this part allows random integrations in Yarrowia lipolytica Po1d strain JMY195 [1] or at a zeta docking platform in Po1d derivative strains like JMY2033 [2] which has the zeta platform at the ura3-302 locus or JMY1212 [3] which has the zeta platform at the leu2-270 locus.


References

[1] Barth G, Gaillardin C. Yarrowia lipolytica. In: Wolf K (ed) Non conventional yeasts in biotechnology. Springer, Berlin (1996) 1, 314-388.

[2] Lazar Z, Rossignol T, Verbeke J, Crutz-Le Coq AM, Nicaud JM, Robak M. Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications. J Ind Microbiol Biotechnol (2013) 40, 1273-83.

[3] Bordes F, Fudalej F, Dossat V, Nicaud JM, Marty A. A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica. J Microbiol Methods (2007) 70, 493-502.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]