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(NADH oxidation assay was carried out using purified FDH protein. 0.1 M sodium phosphate, 0.2mM NADH, 0.1M sodium bicarbonate and 0.2µM FDH were mixed together, and the absorbance was measured at 340nm. Soluble protein fractions from FDH-expressing E.c...) |
(Rokas uploaded a new version of File:NADH oxidation.jpeg) |
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Latest revision as of 21:13, 10 October 2019
NADH oxidation assay was carried out using purified FDH protein. 0.1 M sodium phosphate, 0.2mM NADH, 0.1M sodium bicarbonate and 0.2µM FDH were mixed together, and the absorbance was measured at 340nm. Soluble protein fractions from FDH-expressing E.coli and WT E. coli were used as controls (0.1mg/ml of protein was added to the reaction mixture described above). This assay showed that recombinant FDH_h can be expressed in E.coli and retain functionality.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 21:13, 10 October 2019 |  | 1,231 × 601 (70 KB) | Rokas (Talk | contribs) | NADH oxidation assay was carried out using purified FDH protein. 0.1M pH6.8 sodium phosphate buffer, 0.2mM NADH, 0.1M sodium bicarbonate and 0.2µM FDH were mixed together, and the absorbance was measured at 340nm. Soluble protein fractions from FDH-ex... |
| 18:26, 10 October 2019 |  | 1,231 × 601 (36 KB) | Maria F (Talk | contribs) | NADH oxidation assay was carried out using purified FDH protein. 0.1 M sodium phosphate, 0.2mM NADH, 0.1M sodium bicarbonate and 0.2µM FDH were mixed together, and the absorbance was measured at 340nm. Soluble protein fractions from FDH-expressing E.c... |
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