Difference between revisions of "Part:BBa K2978000:Design"
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===Design Notes===
 
===Design Notes===
−
Need to be express in a chassis that contains the T7 polymerase
+
Needs to be expressed in a chassis with T7 polymerase.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
Synthesized <i>de novo</i> with codon optimization for <i>E.coli</i>. The sequence was obtained from Wang <i>et al.</i> work (2015).  
+
Synthesized <i>de novo</i> with codon optimization for <i>E.coli</i>. The sequence was obtained from Wang <i>et al.</i> (2015) work.
  
 
===References===
 
===References===
 
Wang, Q., Euler, C. W., Delaune, A. & Fischetti, V. A. (2015). Using a Novel Lysin To Help Control <i>Clostridium difficile</i> Infections. <i>Antimicrobial Agents and Chemotherapy</i>, 59(12), 7447–7457. doi:10.1128/aac.01357-15
 
Wang, Q., Euler, C. W., Delaune, A. & Fischetti, V. A. (2015). Using a Novel Lysin To Help Control <i>Clostridium difficile</i> Infections. <i>Antimicrobial Agents and Chemotherapy</i>, 59(12), 7447–7457. doi:10.1128/aac.01357-15

Latest revision as of 18:13, 10 October 2019


Causes cell lysis of the pathogen Clostridium difficile


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needs to be expressed in a chassis with T7 polymerase.

Source

Synthesized de novo with codon optimization for E.coli. The sequence was obtained from Wang et al. (2015) work.

References

Wang, Q., Euler, C. W., Delaune, A. & Fischetti, V. A. (2015). Using a Novel Lysin To Help Control Clostridium difficile Infections. Antimicrobial Agents and Chemotherapy, 59(12), 7447–7457. doi:10.1128/aac.01357-15