Difference between revisions of "Part:BBa K2929001:Experience"
| Line 5: | Line 5: | ||
===Applications of BBa_K2929001=== | ===Applications of BBa_K2929001=== | ||
| + | |||
| + | ==iGEM 2019 St Andrews - Part Development== | ||
| + | <html> | ||
| + | <p>The Mak33 IgG CH3 Domain expresses in well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.</p> | ||
| + | <div align = "center"> | ||
| + | <img src="https://2019.igem.org/wiki/images/2/2c/T--St_Andrews--Antibody_10_Expression_Test.jpg" width="30%"> | ||
| + | <p style="text-align:center;"><font size=-2>Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.</p> | ||
| + | </html> | ||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 14:59, 19 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2929001
iGEM 2019 St Andrews - Part Development
The Mak33 IgG CH3 Domain expresses in well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.
Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.
User Reviews
UNIQ5b59428686b6256a-partinfo-00000001-QINU UNIQ5b59428686b6256a-partinfo-00000002-QINU