Difference between revisions of "Part:BBa K2929001"

 
 
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5' NcoI and 3' BamHI cut site.  
 
5' NcoI and 3' BamHI cut site.  
  
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===Usage and Biology===
 
===Usage and Biology===
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<p>The Mak33 IgG CH3 Domain expresses in well at 25&deg;C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.</p>
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<img src="https://2019.igem.org/wiki/images/2/2c/T--St_Andrews--Antibody_10_Expression_Test.jpg" width="30%">
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<p style="text-align:center;"><font size=-2>Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.</p>
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Latest revision as of 20:01, 19 October 2019


Mak33 IgG CH3 Domain

CH3 Domain of the Mouse (Mus musculus) anticreatine kinase (Mak33) IgG antibody. PDB ID: 1CQK. Codons optimised for Escherichia coli.

5' NcoI and 3' BamHI cut site.


Usage and Biology

The Mak33 IgG CH3 Domain expresses in well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.

Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 66
    Illegal BamHI site found at 322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
  • 1000
    COMPATIBLE WITH RFC[1000]