Difference between revisions of "Part:BBa K3033000"
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This part is a T7 promoter that is inducible with IPTG. Teams could use this sequence as a regulator for the expression of their | This part is a T7 promoter that is inducible with IPTG. Teams could use this sequence as a regulator for the expression of their | ||
| − | desired protein. | + | desired protein. The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase . The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications. |
| + | |||
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Latest revision as of 03:22, 22 October 2019
T7 Promoter
This part is a T7 promoter that is inducible with IPTG. Teams could use this sequence as a regulator for the expression of their desired protein. The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase . The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]