Difference between revisions of "Part:BBa K3033000"

Latest revision as of 03:22, 22 October 2019

T7 Promoter

This part is a T7 promoter that is inducible with IPTG. Teams could use this sequence as a regulator for the expression of their desired protein. The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase . The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3033000 short</partinfo>
-This part is a ribosome binding site which other teams could use for the expression of their desired composite parts.
+This part is a T7 promoter that is inducible with IPTG. Teams could use this sequence as a regulator for the expression of their
+desired protein. The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase . The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.
 <!-- Add more about the biology of this part here