Difference between revisions of "Part:BBa K2938014:Experience"

 
 
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===Applications of BBa_K2938014===
 
===Applications of BBa_K2938014===
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In our plasmids, we placed the deGFP closer to the terminator.
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The presence of the GFP signal proves the expression of the previous subunits on the plasmid and it allows the detection of the transgenic bacteria easily (in mosquitoes)
  
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Here we can see the level of fluorescence of liquid starters from Serratia expressing the different type of plasmid we created. (BBa_K2938014, BBa_K2938015, BBa_K2938016).
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[[File:Fluorescence Plasmids Serratia.png|200px|thumb|left|Fluorescence levels of Serratia expressing our different plasmids]]
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We made toxicity assays, In order to check how effective and toxic are our plasmids.
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We hatched mosquitoes untreated eggs in water containing Serratia bacteria expressing the different plasmids.
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Then we counted the live larva 48 hours after treatment.
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[[File:toxicity_assay.png|200px|thumb|right|Toxicity assay results, Our plasmids show larvicidal activity]]
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===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K2938014 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K2938014 StartReviews</partinfo>

Latest revision as of 10:27, 12 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2938014

In our plasmids, we placed the deGFP closer to the terminator. The presence of the GFP signal proves the expression of the previous subunits on the plasmid and it allows the detection of the transgenic bacteria easily (in mosquitoes)

Here we can see the level of fluorescence of liquid starters from Serratia expressing the different type of plasmid we created. (BBa_K2938014, BBa_K2938015, BBa_K2938016).

Fluorescence levels of Serratia expressing our different plasmids

We made toxicity assays, In order to check how effective and toxic are our plasmids. We hatched mosquitoes untreated eggs in water containing Serratia bacteria expressing the different plasmids. Then we counted the live larva 48 hours after treatment.

Toxicity assay results, Our plasmids show larvicidal activity

User Reviews

UNIQ4043dd01d386c992-partinfo-00000000-QINU UNIQ4043dd01d386c992-partinfo-00000001-QINU