Difference between revisions of "Part:BBa K2936012"

 
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<partinfo>BBa_K2936012 short</partinfo>
 
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AraBAD promoter is an E.coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription
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The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction. araC encodes the AraC protein, which regulates activity of both the PBAD and PC promoters.
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===Usage and Biology===
 
===Usage and Biology===
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===<h1>Experiment</h1>===
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<p>
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  <img src="https://static.igem.org/mediawiki/parts/4/4f/T--ZJUT-China--igem-part12yanse.png" alt=""width="600px">
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<br>
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Figure 1 (a) the pBAD gene of E. coli does include the binding sites of araC plasmid(b)the pBAD gene of E. coli does not include the binding sites of araC plasmid, LacZ alpha gene encodes the β-galactosidase, which could react with ONPG to generate orange color.
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</p>
  
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===Reference===
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[1]Vladislav V Verkhusha, Konstantin A Lukyanov,  (2004) The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. [J]. Nature Biotechnology (2004), 289-296<br>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 06:39, 19 October 2019


araBAD promoter

The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction. araC encodes the AraC protein, which regulates activity of both the PBAD and PC promoters.


Usage and Biology

Experiment


Figure 1 (a) the pBAD gene of E. coli does include the binding sites of araC plasmid(b)the pBAD gene of E. coli does not include the binding sites of araC plasmid, LacZ alpha gene encodes the β-galactosidase, which could react with ONPG to generate orange color.

Reference

[1]Vladislav V Verkhusha, Konstantin A Lukyanov, (2004) The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. [J]. Nature Biotechnology (2004), 289-296
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]