Difference between revisions of "Part:BBa K2967026:Design"

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===Source===
 
===Source===
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None
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They come from the registry.
  
 
===References===
 
===References===
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Merulla, D. & van der Meer, J. R. Regulatable and modulable background expression control in prokaryotic synthetic circuits by auxiliary repressor binding sites. ACS Synth. Biol. 5, 36–45 (2016).

Latest revision as of 06:52, 19 October 2019


The yeaR promoter added an extra NsrRBSs-RBS-Luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 228
    Illegal NgoMIV site found at 1572
    Illegal NgoMIV site found at 1593
    Illegal AgeI site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1478


Design Notes

To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs.

Source

They come from the registry.

References

Merulla, D. & van der Meer, J. R. Regulatable and modulable background expression control in prokaryotic synthetic circuits by auxiliary repressor binding sites. ACS Synth. Biol. 5, 36–45 (2016).