Difference between revisions of "Part:BBa K3041001"
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Coding gene of suckerin-9 from the Humboldt squid <i>Dosidicus gigas</i>, codon optimized for expression in <i>E. coli</i>. | Coding gene of suckerin-9 from the Humboldt squid <i>Dosidicus gigas</i>, codon optimized for expression in <i>E. coli</i>. | ||
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| + | ==Validation== | ||
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| + | The successfully synthesized suckerins, codon-optimized for <em>E.coli</em>, were PCR amplified using the standard biobrick primers. In Figure 1, the PCR products of these specific suckerins were visualized on agarose gel. Suckerin-9, shows strong bands on agarose gel. | ||
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| + | [[File:PCR suckerin proteins.png]] | ||
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| + | <small><b>Figure 1. Amplified suckerin genes 8, 9, and 12.</b> | ||
| + | Polymerase chain reaction (PCR) products amplified with general prefix and suffix primers, shown by gel electrophoresis. Lane 1: 1kb ladder. PCR product at different concentrations of suckerin-8 (408 bp, lane 2 and 3), suckerin-9 (568 bp, lane 4 and 5), and suckerin-12 (696 bp, lane 6 and 7). The gel confirms the amplification of the desired suckerin proteins.</small> | ||
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Latest revision as of 13:45, 18 October 2019
Suckerin-9
Coding gene of suckerin-9 from the Humboldt squid Dosidicus gigas, codon optimized for expression in E. coli.
Validation
The successfully synthesized suckerins, codon-optimized for E.coli, were PCR amplified using the standard biobrick primers. In Figure 1, the PCR products of these specific suckerins were visualized on agarose gel. Suckerin-9, shows strong bands on agarose gel.
Figure 1. Amplified suckerin genes 8, 9, and 12.
Polymerase chain reaction (PCR) products amplified with general prefix and suffix primers, shown by gel electrophoresis. Lane 1: 1kb ladder. PCR product at different concentrations of suckerin-8 (408 bp, lane 2 and 3), suckerin-9 (568 bp, lane 4 and 5), and suckerin-12 (696 bp, lane 6 and 7). The gel confirms the amplification of the desired suckerin proteins.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 527
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]