Difference between revisions of "Part:BBa K3044008:Design"

 
 
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===Design Notes===
 
===Design Notes===
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During the optimization illegal restriction sites were avoided. Aother thing to keep in mind was to figure out wich codons to choose for E. coli K12  
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During the optimization illegal restriction sites were avoided. Another thing to keep in mind was to figure out wich codons to choose for E. coli K12  
  
  

Latest revision as of 15:05, 6 October 2019


dCas9 codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the optimization illegal restriction sites were avoided. Another thing to keep in mind was to figure out wich codons to choose for E. coli K12


Source

The original sequence is from BBa_K1150000 and was codon optimized based on a codon frequency table [1]

References