Difference between revisions of "Part:BBa K3100019"
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<partinfo>BBa_K3100019 short</partinfo> | <partinfo>BBa_K3100019 short</partinfo> | ||
+ | <h2><strong>Usage and Biology:</strong></h2> | ||
+ | KatA is catalase. Microorganisms induce the production of harmful reactive oxygen species (ROS) under acid stress. Overexpression of katA results in a significant decrease in intracellular ROS levels [1]. KatA-mediated ROS clearance plays an important role in conferring resistance to low pH stress in C. glutamicum cells.<br> | ||
− | + | <strong>What's more, we removed the BsaI site by site-specific mutation.</strong> | |
+ | |||
+ | <h2><strong>References:</strong></h2><br> | ||
+ | [1] Xu, N., Lv, H., Wei, L. et al. Impaired oxidative stress and sulfur assimilation contribute to acid tolerance of Corynebacterium glutamicum. Appl Microbiol Biotechnol (2019). | ||
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Latest revision as of 19:08, 21 October 2019
katA(antiacid gene)
Usage and Biology:
KatA is catalase. Microorganisms induce the production of harmful reactive oxygen species (ROS) under acid stress. Overexpression of katA results in a significant decrease in intracellular ROS levels [1]. KatA-mediated ROS clearance plays an important role in conferring resistance to low pH stress in C. glutamicum cells.
What's more, we removed the BsaI site by site-specific mutation.
References:
[1] Xu, N., Lv, H., Wei, L. et al. Impaired oxidative stress and sulfur assimilation contribute to acid tolerance of Corynebacterium glutamicum. Appl Microbiol Biotechnol (2019).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]