Difference between revisions of "Part:BBa K3100019"

 
 
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__NOTOC__
 
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<partinfo>BBa_K3100019 short</partinfo>
 
<partinfo>BBa_K3100019 short</partinfo>
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<h2><strong>Usage and Biology:</strong></h2>
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KatA is catalase. Microorganisms induce the production of harmful reactive oxygen species (ROS) under acid stress. Overexpression of katA results in a significant decrease in intracellular ROS levels [1]. KatA-mediated ROS clearance plays an important role in conferring resistance to low pH stress in C. glutamicum cells.<br>
  
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ybaS(antiacid gene)
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<strong>What's more, we removed the BsaI site by site-specific mutation.</strong>
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<h2><strong>References:</strong></h2><br>
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[1] Xu, N., Lv, H., Wei, L. et al. Impaired oxidative stress and sulfur assimilation contribute to acid tolerance of Corynebacterium glutamicum. Appl Microbiol Biotechnol (2019).
  
 
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Latest revision as of 19:08, 21 October 2019


katA(antiacid gene)

Usage and Biology:

KatA is catalase. Microorganisms induce the production of harmful reactive oxygen species (ROS) under acid stress. Overexpression of katA results in a significant decrease in intracellular ROS levels [1]. KatA-mediated ROS clearance plays an important role in conferring resistance to low pH stress in C. glutamicum cells.

What's more, we removed the BsaI site by site-specific mutation.

References:


[1] Xu, N., Lv, H., Wei, L. et al. Impaired oxidative stress and sulfur assimilation contribute to acid tolerance of Corynebacterium glutamicum. Appl Microbiol Biotechnol (2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]