Difference between revisions of "Part:BBa K3076100:Design"
 
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__NOTOC__
 
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<partinfo>BBa_K2578511 short</partinfo>
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<partinfo>BBa_K3076100 short</partinfo>
  
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<partinfo>BBa_K2578511 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3076100 SequenceAndFeatures</partinfo>
  
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===Source===
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The <i>CgMT</i> sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for <i>E. coli</i> by the ThermoFisher GeneArt platform.
  
 
===Design Notes===
 
===Design Notes===
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This part contains the genomic sequence of CgMT. It was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906) and then codon-optimized for E. coli by ThermoFisher GeneArt platform.
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There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine.
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When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.
  
 
===References===
 
===References===
 
[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064
 
[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064

Latest revision as of 13:41, 13 October 2019


Coding sequence of metallothioneins (MT) gene from Corynebacterium glutamicum (CgMT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The CgMT sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for E. coli by the ThermoFisher GeneArt platform.

Design Notes

There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine. When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.

References

[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064