Difference between revisions of "Part:BBa K3076200:Design"

 
 
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<partinfo>BBa_K3076250 short</partinfo>
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<partinfo>BBa_K3076200 short</partinfo>
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<partinfo>BBa_K3076250 SequenceAndFeatures</partinfo>
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===Design Notes===
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Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.
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<partinfo>BBa_K3076200 SequenceAndFeatures</partinfo>
  
  
 
===Source===
 
===Source===
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This part contains the coding sequence of the <i>vanabin2</i> gene. It was identified from the literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon-optimized for <i>E. coli</i> by the ThermoFisher GeneArt platform.
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This part contains the coding sequence of vanabin2 gene. It was identified from literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon optimized for E. coli by ThermoFisher GeneArt platform.  
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===Design notes===
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Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.
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===References===
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===Reference===
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[1] Ueki, T., Sakamoto, Y., Yamaguchi, N., & Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian <i>Ascidia sydneiensis samea</i>. Applied and Environmental Microbiology, 69(11), 6442–6446. doi: 10.1128/aem.69.11.6442-6446.2003
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Latest revision as of 12:48, 11 October 2019


Coding sequence of Vanabin2 gene from Ascidia sydneiensis samea


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This part contains the coding sequence of the vanabin2 gene. It was identified from the literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon-optimized for E. coli by the ThermoFisher GeneArt platform.

Design notes

Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.

Reference

[1] Ueki, T., Sakamoto, Y., Yamaguchi, N., & Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian Ascidia sydneiensis samea. Applied and Environmental Microbiology, 69(11), 6442–6446. doi: 10.1128/aem.69.11.6442-6446.2003