Difference between revisions of "Part:BBa K2938004:Design"
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===References=== | ===References=== | ||
[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015. | [1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015. | ||
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[2] Y. Xu, M. Nagai, M. Bagdasarian, T. W. Smith, and E. D. Walker, “Expression of the p20 Gene from Bacillus thuringiensis H-14 Increases Cry11A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria,” Appl. Environ. Microbiol., vol. 67, no. 7, pp. 3010–3015, Jul. 2001. | [2] Y. Xu, M. Nagai, M. Bagdasarian, T. W. Smith, and E. D. Walker, “Expression of the p20 Gene from Bacillus thuringiensis H-14 Increases Cry11A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria,” Appl. Environ. Microbiol., vol. 67, no. 7, pp. 3010–3015, Jul. 2001. | ||
Latest revision as of 13:21, 4 October 2019
P20
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 264
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 264
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 264
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 264
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 264
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
optimized for E.Coli
Source
Isolated from pVE4-ADRC [1]plasmid by PCR.
References
[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.
[2] Y. Xu, M. Nagai, M. Bagdasarian, T. W. Smith, and E. D. Walker, “Expression of the p20 Gene from Bacillus thuringiensis H-14 Increases Cry11A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria,” Appl. Environ. Microbiol., vol. 67, no. 7, pp. 3010–3015, Jul. 2001.