Difference between revisions of "Part:BBa K137068:Experience"

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===Applications of BBa_K137068===
 
===Applications of BBa_K137068===
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*KatG+LAA is functional
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====KatG+LAA is functional====
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**Strain: SN0037 (DE katE, katG)
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*Construct: K137079
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**Media: M9 + 2% glucose
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*Strain: SN0037 (ΔkatE, katG)
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**KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
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*Media: M9 + 2% glucose
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*KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
  
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<center>
 
{| border="1"
 
{| border="1"
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!Hydrogen peroxide (mM)!! +AHL (100mM)!! -AHL
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!Hydrogen peroxide (mM)!! +AHL (100nM)!! -AHL
  
 
|-
 
|-
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|0.0|| Y || Y  
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!0.0
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| style="color:green" | Y || style="color:green" | Y  
 
|-
 
|-
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|0.3|| Y || Y
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!0.3
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| style="color:green" | Y || style="color:green" | Y
 
|-
 
|-
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|1.0|| Y || N
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!1.0
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| style="color:green" | Y || style="color:red" | N
 
|-
 
|-
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|3.0|| Y || N
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!3.0
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| style="color:green" | Y || style="color:red" | N
 
|-
 
|-
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|10.0|| N || N
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!10.0
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| style="color:red" | N || style="color:red" | N
 
|}
 
|}
  
 
Y = Turbid growth after overnight incubation.
 
Y = Turbid growth after overnight incubation.
 
N = No turbid growth after overnight incubation.
 
N = No turbid growth after overnight incubation.
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</center>
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 00:13, 5 September 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K137068

KatG+LAA is functional

  • Construct: K137079
  • Strain: SN0037 (ΔkatE, katG)
  • Media: M9 + 2% glucose
  • KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.


Hydrogen peroxide (mM) +AHL (100nM) -AHL
0.0 Y Y
0.3 Y Y
1.0 Y N
3.0 Y N
10.0 N N

Y = Turbid growth after overnight incubation. N = No turbid growth after overnight incubation.

User Reviews

UNIQ9d7baec1844b4280-partinfo-00000000-QINU UNIQ9d7baec1844b4280-partinfo-00000001-QINU