Difference between revisions of "Part:BBa K2967026:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs. | |
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===Source=== | ===Source=== | ||
| − | + | They come from the registry. | |
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===References=== | ===References=== | ||
| + | Merulla, D. & van der Meer, J. R. Regulatable and modulable background expression control in prokaryotic synthetic circuits by auxiliary repressor binding sites. ACS Synth. Biol. 5, 36–45 (2016). | ||
Latest revision as of 06:52, 19 October 2019
The yeaR promoter added an extra NsrRBSs-RBS-Luc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 228
Illegal NgoMIV site found at 1572
Illegal NgoMIV site found at 1593
Illegal AgeI site found at 1296 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1478
Design Notes
To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs.
Source
They come from the registry.
References
Merulla, D. & van der Meer, J. R. Regulatable and modulable background expression control in prokaryotic synthetic circuits by auxiliary repressor binding sites. ACS Synth. Biol. 5, 36–45 (2016).