Difference between revisions of "Part:BBa K2967019"

Latest revision as of 12:51, 10 October 2019

The yeaR promoter will initiate the express of IL-10 when nitric oxide appear

In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting. It has been proved that the expression level can be induced when adding nitric oxide.

Figure 1. Diagram of expressing vector in backbone pCDFDuet-1. Promoter yeaR is an nitric oxide inducible promoter. Gene hIL-10 is one of our target protein.

Figure 2. Protein expressing of human il-10 gene which is transformed into BL21 strain and induced by nitric oxide. After induction of nitric oxide, the culture is incubated at 37℃ for 2 hours. Western blotting demonstrats the expression level of hIL-10. In order to equate the total number of cells in each lane, dilution is made to keep each sample at the same OD and sample volume.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2967019 short</partinfo>
-In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting to show the result. It has been proved that the expression level can be induced when adding nitric oxide.
+In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting. It has been proved that the expression level can be induced when adding nitric oxide.
 https://static.igem.org/mediawiki/parts/thumb/7/7e/T--NEU_China--part--pyear-il10-1.png/800px-T--NEU_China--part--pyear-il10-1.png
+'''Figure 1. Diagram of expressing vector in backbone pCDFDuet-1.''' Promoter yeaR is an nitric oxide inducible promoter. Gene hIL-10 is one of our target protein.
 https://static.igem.org/mediawiki/parts/thumb/3/3c/T--NEU_China--part--pyear%2Bli10-2.png/800px-T--NEU_China--part--pyear%2Bli10-2.png
+'''Figure 2. Protein expressing of human il-10 gene which is transformed into BL21 strain and induced by nitric oxide.''' After induction of nitric oxide, the culture is incubated at 37℃ for 2 hours. Western blotting demonstrats the expression level of hIL-10. In order to equate the total number of cells in each lane, dilution is made to keep each sample at the same OD and sample volume.
 <!-- Add more about the biology of this part here