Difference between revisions of "Part:BBa K2996501:Design"
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===References=== | ===References=== | ||
| − | [1]Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012) | + | [1]Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012); |
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[2]Burz, DS (Burz, DS); Rivera-Pomar, R (Rivera-Pomar, R); Jackle, H (Jackle, H); Hanes, SD (Hanes, SD).Cooperative DNA-binding by Bicoid provides a mechanism for threshold-dependent gene activation in the Drosophila embryo. EMBO JOURNAL 17, 5998-6009(1998) | [2]Burz, DS (Burz, DS); Rivera-Pomar, R (Rivera-Pomar, R); Jackle, H (Jackle, H); Hanes, SD (Hanes, SD).Cooperative DNA-binding by Bicoid provides a mechanism for threshold-dependent gene activation in the Drosophila embryo. EMBO JOURNAL 17, 5998-6009(1998) | ||
Latest revision as of 07:03, 3 October 2019
Terminator put downstream of zinc finger
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 304
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted a relatively strong terminator that can stop the transcription of DBD protein,thus lowering the leakage in the system,so we chose the terminator BBa_B0010 as the terminator.We designed new PCR primers to extend the original DNA sequence of DBD protein(BBa_K2996500),adding the DNA sequence of BBa_B0010 to the sequence.
Source
The subparts of this composite part were BBa_K2996500 and BBa_B0010.BBa_B0010 was added through one additional PCR on the basis of BBa_K2996500.
References
[1]Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012);
[2]Burz, DS (Burz, DS); Rivera-Pomar, R (Rivera-Pomar, R); Jackle, H (Jackle, H); Hanes, SD (Hanes, SD).Cooperative DNA-binding by Bicoid provides a mechanism for threshold-dependent gene activation in the Drosophila embryo. EMBO JOURNAL 17, 5998-6009(1998)