Difference between revisions of "Part:BBa K3221001:Design"

Latest revision as of 06:34, 1 October 2019

CNS3

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 2614
Illegal SpeI site found at 1275
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1303
Illegal SpeI site found at 1275
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1747
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 2614
Illegal SpeI site found at 1275
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 2614
Illegal SpeI site found at 1275
Illegal AgeI site found at 2677
1000
COMPATIBLE WITH RFC[1000]

Design Notes

There are two domains in this parts, NK and HisG. They are relatively independent, so we can devide them by PCR.

Source

We got this part by gene synthesis. And it can also be found in the genome of Cordyceps militaris.

References

Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.

Revision as of 05:50, 1 October 2019 (view source) Registry (Talk \| contribs)		Latest revision as of 06:34, 1 October 2019 (view source) Jinqihan (Talk \| contribs) (→‎References)
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	===References===		===References===
		+	Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.