Difference between revisions of "Part:BBa K3111032:Design"
(→References) |
|||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | <partinfo> | + | <partinfo>BBa_K3111032 short</partinfo> |
| − | <partinfo> | + | <partinfo>BBa_K3111032 SequenceAndFeatures</partinfo> |
===Design Notes=== | ===Design Notes=== | ||
| − | 15aa | + | Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | MiniSOG was derived from Arabidopsis thaliana. T. maritima targeting peptide taken from Cassidy-Amstutz et al, 2016. | |
===References=== | ===References=== | ||
| + | |||
| + | Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, et al. Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry. 2016 Jun 21;55(24):3461– | ||
Latest revision as of 11:36, 14 October 2019
Photosensitiser miniSOG fused to T. maritima encapsulin loading peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 346
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016.
Source
MiniSOG was derived from Arabidopsis thaliana. T. maritima targeting peptide taken from Cassidy-Amstutz et al, 2016.
References
Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, et al. Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry. 2016 Jun 21;55(24):3461–