Difference between revisions of "Part:BBa K112201:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | <pre> | ||
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) | PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) | ||
Sub into pBca1256 (EcoRI/BamHI) | Sub into pBca1256 (EcoRI/BamHI) | ||
| Line 14: | Line 15: | ||
bx2 Reverse biobricking of {<T4 Holin!} | bx2 Reverse biobricking of {<T4 Holin!} | ||
cgttaGGATCCttatttagcccttcctaatattctggccgcc | cgttaGGATCCttatttagcccttcctaatattctggccgcc | ||
| + | </pre> | ||
===Source=== | ===Source=== | ||
Latest revision as of 23:31, 3 September 2008
< T4 holin! in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3 Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2 Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc