Difference between revisions of "Part:BBa K112002:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K112002 short</partinfo> | <partinfo>BBa_K112002 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | <pre> | |
| − | + | PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) | |
| − | + | Sub into pBca1256 (EcoRI/BamHI) | |
| − | + | Product is pBca1256-K112002 | |
| + | ---------------------------------------- | ||
| + | bx1 Forward biobricking of {T4 Holin>} | ||
| + | ccagtGAATTCatgAGATCTatggcagcacctagaatatc | ||
| + | bx4 Reverse biobricking of {T4 Holin>} | ||
| + | cgttaGGATCCtttagcccttcctaatattctggcc | ||
| + | </pre> | ||
===Source=== | ===Source=== | ||
Latest revision as of 23:32, 3 September 2008
T4 holin, without stop codon, berkeley standard
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1 Forward biobricking of {T4 Holin>}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx4 Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc