Difference between revisions of "Part:BBa K2973015"

 
 
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<partinfo>BBa_K2973015 short</partinfo>
 
<partinfo>BBa_K2973015 short</partinfo>
  
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Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic disease.  
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<i>Hepatitis B</i> is a DNA virus that attacks the liver and can cause both acute and chronic diseases.  
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This part represents a small fragment of the virus' genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.  
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This part represents a small fragment of the virus's genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfully amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).
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PCR Reaction:
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<html>
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  <head>
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      <title>HTML img Tag</title>
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  </head>
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  <body>
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      <img src="https://static.igem.org/mediawiki/parts/5/59/Thessaly_2019_HBV_PCR_Gel.jpeg" width="200"
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        height="300">
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  </body>
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</html>
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 +
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RPA reaction after cleanup of the amplified product:
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 +
<html>
 +
  <head>
 +
      <title>HTML img Tag</title>
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  </head>
 +
 +
  <body>
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      <img src="https://2019.igem.org/wiki/images/a/af/T--Thessaly--Results_Figure9.jpg" width="200"
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        height="300">
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  </body>
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</html>
  
 
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Latest revision as of 12:58, 21 October 2019


Hepatitis B Virus fragment (281-560bp)

Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic diseases. This part represents a small fragment of the virus's genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.

Usage and Biology

This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfully amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).

PCR Reaction:

HTML img Tag


RPA reaction after cleanup of the amplified product:

HTML img Tag

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]