Difference between revisions of "Part:BBa K3064010"
 
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<partinfo>BBa_K3064010 short</partinfo>
 
<partinfo>BBa_K3064010 short</partinfo>
  
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It is a known type of E3 ubiquitin-protein ligase as an intracellular antibody effector in the antibody-mediated proteolysis pathway.
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Trim 21 comes from the family of TRIM. ‘MMu’ means that it is from mouse. It is a known type of E3 ubiquitin-protein ligase as an intracellular antibody effector in the antibody-mediated proteolysis pathway.
  
 
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Due to the organism, therapeutic antibodies targeting disease-associated antigens in extracellular is a key tool for some certain diseases in current researches. Although some diseases is not caused by virons, trim 21 can be used in these diseased by blocking a particular pathogenic process.<Br/>
 
Due to the organism, therapeutic antibodies targeting disease-associated antigens in extracellular is a key tool for some certain diseases in current researches. Although some diseases is not caused by virons, trim 21 can be used in these diseased by blocking a particular pathogenic process.<Br/>
  
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[[File:HA-tag--mmuTrim21.png|130px|]]
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===Result===
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To validate the protein degradation efficiency of the ScFv-based ErbB3 Predator system, we
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transfected MCF7 cells with plasmids expressing ScFvErbB3-linker-Fc fusion protein and HA-
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Trim21. Western Blotting analysis showed that with the high expression of ScFvErbB3-linker-
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Fc fusion protein and HA-Trim21, the ErbB3 protein level decreased dramatically in cells
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transfected with ErbB3 Predator (approximately 30%, Fig 1A and B). This result demonstrated that the ScFv-based Predator system could successfully degrade the targeted membrane protein.
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https://static.igem.org/mediawiki/parts/c/c6/T--NUDT_CHINA--erbB3-composite.jpg
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Figure 1. (A and B) Western blotting assay showing the components of ErbB3 Predator and the degradation of ErbB3.
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[[File:Trim21.png|130px|]]
 
  
 
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===Sequence and Features===
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3064010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3064010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3064010 parameters</partinfo>
 
<partinfo>BBa_K3064010 parameters</partinfo>
 
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===References===
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1.TRIM21[G/OL]. https://en.wikipedia.org/wiki/TRIM21 ,2019.
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2.Stian Foss, Ruth Watkinson, Inger Sandlie, et al. TRIM21: a cytosolic Fc receptor with broad antibody isotype specificity[J]. Immunological Reviews, 2015, 268(1):328-339.
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3.Lee AYS. A review of the role and clinical utility of anti-Ro52/TRIM21 in systemic autoimmunity[J]. Rheumatology International, 2017, 37(8):1323-1333.

Latest revision as of 00:08, 27 October 2020


mmuTrim21

Trim 21 comes from the family of TRIM. ‘MMu’ means that it is from mouse. It is a known type of E3 ubiquitin-protein ligase as an intracellular antibody effector in the antibody-mediated proteolysis pathway.

Usage and Biology

Trim 21 has a particular domain to recognize the Fc domain and combine with antibody marked no-enveloped virions. Using its E3 ubiquitin-protein ligase activity, trim 21 with the antibody bond to virons is degraded by the Ubiquitin dependent protease degradation pathway.

Due to the organism, therapeutic antibodies targeting disease-associated antigens in extracellular is a key tool for some certain diseases in current researches. Although some diseases is not caused by virons, trim 21 can be used in these diseased by blocking a particular pathogenic process.

Result

To validate the protein degradation efficiency of the ScFv-based ErbB3 Predator system, we transfected MCF7 cells with plasmids expressing ScFvErbB3-linker-Fc fusion protein and HA- Trim21. Western Blotting analysis showed that with the high expression of ScFvErbB3-linker- Fc fusion protein and HA-Trim21, the ErbB3 protein level decreased dramatically in cells transfected with ErbB3 Predator (approximately 30%, Fig 1A and B). This result demonstrated that the ScFv-based Predator system could successfully degrade the targeted membrane protein.

T--NUDT_CHINA--erbB3-composite.jpg

Figure 1. (A and B) Western blotting assay showing the components of ErbB3 Predator and the degradation of ErbB3.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 75
    Illegal BamHI site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 176
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1.TRIM21[G/OL]. https://en.wikipedia.org/wiki/TRIM21 ,2019.

2.Stian Foss, Ruth Watkinson, Inger Sandlie, et al. TRIM21: a cytosolic Fc receptor with broad antibody isotype specificity[J]. Immunological Reviews, 2015, 268(1):328-339.

3.Lee AYS. A review of the role and clinical utility of anti-Ro52/TRIM21 in systemic autoimmunity[J]. Rheumatology International, 2017, 37(8):1323-1333.