Difference between revisions of "Part:BBa K3064022"
 
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===Usage and Biology===
 
===Usage and Biology===
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HA-tag, derived from the HA-molecule corresponding to amino acids 98-106, is used as a general epitope tag in expression vectors. It can facilitate the detection, isolation and purification of target proteins.<Br/>
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HA-tag, derived from the HA-molecule corresponding to amino acids 98-106, is used in expression vectors as a epitope tag. It can be used in the detection, isolation and purification of target proteins.<Br/>
  
 
Trim 21 belongs the family of TRIM and exist in many eukaryotic cells. MmuTrim 21 comes from mouse eukaryotic cells. It can bind IgG, IgM, IgA as a cytosolic Fc receptor. In intracellular cell, Trim 21 can orchestrates humoral immunity in the cytosolic environment and mediates neutralization by degrading antibody-coated viruses in the proteasome as a E3 ubiquitin-protein ligase.<Br/>
 
Trim 21 belongs the family of TRIM and exist in many eukaryotic cells. MmuTrim 21 comes from mouse eukaryotic cells. It can bind IgG, IgM, IgA as a cytosolic Fc receptor. In intracellular cell, Trim 21 can orchestrates humoral immunity in the cytosolic environment and mediates neutralization by degrading antibody-coated viruses in the proteasome as a E3 ubiquitin-protein ligase.<Br/>
  
 
This composite parts can be used to test the degradation of  some certain targeting proteins, antibody-antigen complex and receptor-ligand complex with more convenience.<Br/>
 
This composite parts can be used to test the degradation of  some certain targeting proteins, antibody-antigen complex and receptor-ligand complex with more convenience.<Br/>
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[[File:HA-tag--mmuTrim21.png|130px|]]
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[[File:Trim21.png|130px|]]
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===Special Design===
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HA-tag can test targeting protein eaily. So this composite part is detected more simply.
  
 
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<partinfo>BBa_K3064022 parameters</partinfo>
 
<partinfo>BBa_K3064022 parameters</partinfo>
 
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===References===
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1.Stian Foss, Ruth Watkinson, Inger Sandlie, et al. TRIM21: a cytosolic Fc receptor with broad antibody isotype specificity[J]. Immunological Reviews, 2015, 268(1):328-339.
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2.William A. McEwan, Leo C. James. Progress in Molecular Biology and Translational Science, 2015, 129:167-187.
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3.HA-tag[G/OL]. https://en.wikipedia.org/wiki/HA-tag , 2019.

Latest revision as of 14:34, 29 September 2019


HA-tag->mmuTrim21

This part can be used to test some certain expressed proteins that can bind with mmuTrim21.

Usage and Biology

HA-tag, derived from the HA-molecule corresponding to amino acids 98-106, is used in expression vectors as a epitope tag. It can be used in the detection, isolation and purification of target proteins.

Trim 21 belongs the family of TRIM and exist in many eukaryotic cells. MmuTrim 21 comes from mouse eukaryotic cells. It can bind IgG, IgM, IgA as a cytosolic Fc receptor. In intracellular cell, Trim 21 can orchestrates humoral immunity in the cytosolic environment and mediates neutralization by degrading antibody-coated viruses in the proteasome as a E3 ubiquitin-protein ligase.

This composite parts can be used to test the degradation of some certain targeting proteins, antibody-antigen complex and receptor-ligand complex with more convenience.

HA-tag--mmuTrim21.png

Trim21.png

Special Design

HA-tag can test targeting protein eaily. So this composite part is detected more simply.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 110
    Illegal BamHI site found at 813
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1.Stian Foss, Ruth Watkinson, Inger Sandlie, et al. TRIM21: a cytosolic Fc receptor with broad antibody isotype specificity[J]. Immunological Reviews, 2015, 268(1):328-339.

2.William A. McEwan, Leo C. James. Progress in Molecular Biology and Translational Science, 2015, 129:167-187.

3.HA-tag[G/OL]. https://en.wikipedia.org/wiki/HA-tag , 2019.