Difference between revisions of "Part:BBa K2938002"
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===Amplification===
 
===Amplification===
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[[File:Cry4Ba PCR.jpeg|200px|thumb|left|PCR  amplification of Cry4Ba by PCR.]]
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[[File:Cry4Ba PCR.jpeg|200px|thumb|left|PCR  amplification of Cry4Ba]]
  
  

Latest revision as of 12:42, 17 October 2019


Cry4Ba

Description

Pesticidal crystal protein Cry4Ba is a 128 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). The pBtoxis is a toxin-encoding plasmid that its origin is the Bti and it encodes to four Cry toxins (Cry4Aa, Cry4Ba, Cry10Aa and Cry11Aa) and three Cyt toxins (Cyt1Aa, Cyt2Ba and Cyt1Ca)[1], which are specifically toxic against Aedes, Anopheles and Culex mosquitoes which are vectors to many diseases caused by parasites and viruses, such as Malaria, Zika and Dengue. Each of the toxins hold a low level of larvicidal effect but their combination has a synergetic effect which is stronger than the linear combination we expect. The Cyt1Aa toxin effect in the synergetic effect is mainly the prevention of resistance in the mosquito to the Cry toxins. The bigger Cry toxins, Cry4Aa and Cry4Ba have a conserved C-terminal end which helps the toxin fold correctly and form crystal structures.

ToxinTable.png

Cry4Ba is toxic specifically against Aedes Aegypti mosquitoes’ larvae (compared to Cry4Aa) [2]


Amplification

PCR amplification of Cry4Ba


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3
    Illegal XbaI site found at 1681
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal NheI site found at 69
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3
    Illegal XbaI site found at 1681
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3
    Illegal XbaI site found at 1681
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
    Illegal NgoMIV site found at 2493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475