Difference between revisions of "Part:BBa K3044006"

 
 
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<partinfo>BBa_K3044006 short</partinfo>
 
<partinfo>BBa_K3044006 short</partinfo>
  
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This part make bacteria express a clear green color. This plasmid can be useful if one want to follow the plasmid's transmission from one bacteria to another or it can be used to validate the expression of a gene that can be inserted before the GFP coding sequence.
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This part make bacteria express the GFP protein.
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The SDU-Denmark 2019 team used this part to create an ''E. coli'' that expressed GFP. A plasmid containing a (d)Cas9/sgRNA system were transformed into this chassis, and by fluorescence microscopy and FACS it was validated whether the (d)Cas9/sgRNA could downregulate the expression of GFP.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:58, 22 October 2019


Strong constitutively active Anderson promoter (0.86) with a GFP coding sequence

This part make bacteria express the GFP protein. The SDU-Denmark 2019 team used this part to create an E. coli that expressed GFP. A plasmid containing a (d)Cas9/sgRNA system were transformed into this chassis, and by fluorescence microscopy and FACS it was validated whether the (d)Cas9/sgRNA could downregulate the expression of GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705