Difference between revisions of "Part:BBa K3113102:Design"
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===Design Notes=== | ===Design Notes=== | ||
Cloning Primers: | Cloning Primers: | ||
| + | |||
fw | fw | ||
AATTCGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCGGCGGGAGTGCTAGCAGCCCGGAAGATGAAAACCAGAGCCTGGAACAGAAAAACAGCCAGCTGAAACAGGAAATTAGCCAGCTGGAACAGGAAATTCAGCAGCTGGAATATTGAA | AATTCGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCGGCGGGAGTGCTAGCAGCCCGGAAGATGAAAACCAGAGCCTGGAACAGAAAAACAGCCAGCTGAAACAGGAAATTAGCCAGCTGGAACAGGAAATTCAGCAGCTGGAATATTGAA | ||
rev | rev | ||
CGCGTTCAATATTCCAGCTGCTGAATTTCCTGTTCCAGCTGGCTAATTTCCTGTTTCAGCTGGCTGTTTTTCTGTTCCAGGCTCTGGTTTTCATCTTCCGGGCTGCTAGCACTCCCGCCGCTAATCTTCTTGAACAGCCGCCAGCCGCTCACG | CGCGTTCAATATTCCAGCTGCTGAATTTCCTGTTCCAGCTGGCTAATTTCCTGTTTCAGCTGGCTGTTTTTCTGTTCCAGGCTCTGGTTTTCATCTTCCGGGCTGCTAGCACTCCCGCCGCTAATCTTCTTGAACAGCCGCCAGCCGCTCACG | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Latest revision as of 08:41, 26 September 2019
P9SN
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Cloning Primers:
fw AATTCGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCGGCGGGAGTGCTAGCAGCCCGGAAGATGAAAACCAGAGCCTGGAACAGAAAAACAGCCAGCTGAAACAGGAAATTAGCCAGCTGGAACAGGAAATTCAGCAGCTGGAATATTGAA rev CGCGTTCAATATTCCAGCTGCTGAATTTCCTGTTCCAGCTGGCTAATTTCCTGTTTCAGCTGGCTGTTTTTCTGTTCCAGGCTCTGGTTTTCATCTTCCGGGCTGCTAGCACTCCCGCCGCTAATCTTCTTGAACAGCCGCCAGCCGCTCACG
Source
This sequence was provided by our supervisor PhD student Susanne Pettinger, Westmeyer Lab, Helmholtz Center Munich.
Ljubetič, A., et al. (2017). "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo." Nature Biotechnology 35: 1094. https://www.nature.com/articles/nbt.3994#s1, Supplementary Text and Figures