Difference between revisions of "Part:BBa K2916010"
 
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Creatine kinase is a protein that is used in the PURE and OnePot PURE cell-free systems.
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Creatine kinase is a protein that is used in the OnePot PURE cell-free system.
  
 
<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
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===Usage and Biology===
 
===Usage and Biology===
  
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Used in OnePot PURE
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CK participates in aminoacyl-tRNA based protein biosynthesis as a reaction catalysing enzyme.
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In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.
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For our project we used CK as a part of the protein solution needed for <html><a style="padding: 0px; margin: 0px;" href="https://2019.igem.org/Team:EPFL/OnePot_Pure"> OnePot PURE cell-free system </a></html> produced with the method of gravity flow affinity chromatography, as described in the <html><a style="padding: 0px; margin: 0px;" href="https://www.protocols.io/view/protein-purification-for-onepot-pure-cell-free-sys-8auhsew"> protocol </a></html> we designed.
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===Characterization===
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All the data regarding the characterization of that part can be found in the composite part: <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K2916045"> BBa_K2916045 </a></html>.
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2916010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2916010 SequenceAndFeatures</partinfo>

Latest revision as of 03:19, 22 October 2019


CK protein equipped with a 6x HIS affinity tag


Creatine kinase is a protein that is used in the OnePot PURE cell-free system.


Usage and Biology

CK participates in aminoacyl-tRNA based protein biosynthesis as a reaction catalysing enzyme.

In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.


For our project we used CK as a part of the protein solution needed for OnePot PURE cell-free system produced with the method of gravity flow affinity chromatography, as described in the protocol we designed.


Characterization

All the data regarding the characterization of that part can be found in the composite part: BBa_K2916045 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 821
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172