Difference between revisions of "Part:BBa K2916045:Design"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2916044 short</partinfo>
+
<partinfo>BBa_K2916045 short</partinfo>
  
−
<partinfo>BBa_K2916044 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2916045 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
f
 
−
 
  
 +
The protein was expressed in pQE30 in M15 E.coli strain.
  
 
===Source===
 
===Source===
  
−
f
+
Sebastian Maerkl Lab at EPFL, Switzerland.
 +
 
 +
http://www.addgene.org/124134/
  
 
===References===
 
===References===
 +
<I>
 +
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
 +
 +
Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.</I>

Latest revision as of 03:17, 22 October 2019


Expression of CK in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 974
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein was expressed in pQE30 in M15 E.coli strain.

Source

Sebastian Maerkl Lab at EPFL, Switzerland.

http://www.addgene.org/124134/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.