Difference between revisions of "Part:BBa K2916040:Design"
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__NOTOC__ | __NOTOC__ | ||
− | <partinfo> | + | <partinfo>BBa_K2916040 short</partinfo> |
− | <partinfo> | + | <partinfo>BBa_K2916040 SequenceAndFeatures</partinfo> |
===Design Notes=== | ===Design Notes=== | ||
− | |||
− | |||
+ | The protein was expressed in pQE30 in M15 E.coli strain. | ||
===Source=== | ===Source=== | ||
− | + | Sebastian Maerkl Lab at EPFL, Switzerland. | |
+ | |||
+ | https://www.addgene.org/124129/ | ||
===References=== | ===References=== | ||
+ | <I> | ||
+ | Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568 | ||
+ | |||
+ | Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.</I> |
Latest revision as of 03:00, 22 October 2019
Expression of RF1 in E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 712
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The protein was expressed in pQE30 in M15 E.coli strain.
Source
Sebastian Maerkl Lab at EPFL, Switzerland.
https://www.addgene.org/124129/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.