Difference between revisions of "Part:BBa K2916035:Design"

 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2916034 short</partinfo>
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<partinfo>BBa_K2916035 short</partinfo>
  
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<partinfo>BBa_K2916034 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2916035 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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The protein was expressed in pQE30 in M15 E.coli strain.
  
 
===Source===
 
===Source===
  
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Sebastian Maerkl Lab at EPFL, Switzerland.
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http://www.addgene.org/124124/
  
 
===References===
 
===References===
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<i>Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
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Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.</i>

Latest revision as of 02:26, 22 October 2019


Expression of IF2 in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2405
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1760
    Illegal AgeI site found at 371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1091
    Illegal SapI site found at 1026


Design Notes

The protein was expressed in pQE30 in M15 E.coli strain.

Source

Sebastian Maerkl Lab at EPFL, Switzerland.

http://www.addgene.org/124124/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.