Difference between revisions of "Part:BBa K3105667"

 
 
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<partinfo>BBa_K3105667 short</partinfo>
 
<partinfo>BBa_K3105667 short</partinfo>
  
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Coding sequence of aryl-alcohol oxidase (AAO) codon optimised for Pichia pastoris
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Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for <I>Pichia pastoris</I>
  
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In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide.
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In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).
  
 
AAO facilitates following reaction:
 
AAO facilitates following reaction:
  
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primary aromatic alcohol + O2 = aromatic aldehyde + H2O2
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primary aromatic alcohol + O<sub>2</sub> = aromatic aldehyde + H<sub>2</sub>O<sub>2</sub>
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<br><br><br>
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<b>References:</b> <br>
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Ferreira, P. et al. (2009) ‘Aryl-alcohol oxidase involved in lignin degradation. A mechanistic study based on steady and pre-steady state kinetics and primary and solvent isotope effects with two alcohol substrates’, Journal of Biological Chemistry. doi: 10.1074/jbc.M109.011593.
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<br><br>
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 01:13, 21 October 2019


Aryl-alcohol oxidase

Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for Pichia pastoris

In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).

AAO facilitates following reaction:

primary aromatic alcohol + O2 = aromatic aldehyde + H2O2


References:
Ferreira, P. et al. (2009) ‘Aryl-alcohol oxidase involved in lignin degradation. A mechanistic study based on steady and pre-steady state kinetics and primary and solvent isotope effects with two alcohol substrates’, Journal of Biological Chemistry. doi: 10.1074/jbc.M109.011593.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 150
    Illegal BamHI site found at 337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1297
  • 1000
    COMPATIBLE WITH RFC[1000]