Difference between revisions of "Part:BBa K2916011:Design"

 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2916010 short</partinfo>
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<partinfo>BBa_K2916011 short</partinfo>
  
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<partinfo>BBa_K2916010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2916011 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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The sequence was extracted from the pET21a-IleRS-His plasmid and expressed in the BL21 (DE3) E.coli strain.
 
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Codon optimization : 1179 from G to C
 
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Second stop codon was added in compliance with iGEM standards.
 
  
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The sequence was extracted from the pET-21a(+)-AK1-His plasmid and expressed in the BL21 (DE3) E.coli strain.
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 +
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Second stop codon was added in compliance with iGEM standards.
  
  
 
===Source===
 
===Source===
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Sebastian Maerkl Lab at EPFL, Switzerland.  
 
Sebastian Maerkl Lab at EPFL, Switzerland.  
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http://www.addgene.org/124112/  
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http://www.addgene.org/118977/  
  
 
===References===
 
===References===
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<I>
 +
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Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
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Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.
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</I>

Latest revision as of 03:27, 22 October 2019


MK protein equipped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 583
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403
    Illegal BsaI.rc site found at 433


Design Notes

The sequence was extracted from the pET-21a(+)-AK1-His plasmid and expressed in the BL21 (DE3) E.coli strain.


Second stop codon was added in compliance with iGEM standards.


Source

Sebastian Maerkl Lab at EPFL, Switzerland.

http://www.addgene.org/118977/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.