Difference between revisions of "Part:BBa K2927002"
 
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<partinfo>BBa_K2927002 short</partinfo>
 
<partinfo>BBa_K2927002 short</partinfo>
  
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10 times histidine is made up of 10 connected histidines. Because of the affinity with Ni, it makes the composition (10X His-MBP-TEV-Cas12a) bind on the Ni column. It’s used for Cas12a protein purification.  
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This sequence encoded 10 connected histidines, which can be used for protein purification through strong interaction with Nickel (II) ion. It belongs to the compositions of Cas12a expression plasmid (10X His-MBP-TEV-Cas12a).  
  
 
'''Reference'''<br>
 
'''Reference'''<br>

Latest revision as of 04:38, 16 October 2019

10X His

This sequence encoded 10 connected histidines, which can be used for protein purification through strong interaction with Nickel (II) ion. It belongs to the compositions of Cas12a expression plasmid (10X His-MBP-TEV-Cas12a).

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]