Difference between revisions of "Part:BBa K3168008"

Latest revision as of 15:27, 21 October 2019

HisTag-MBP-TEVsite

This basic part includes a His-tag, maltose-binding protein (MBP) and a TEV protease recognition site. The His-tag can be used for protein purification with Immobilized Metal Affinity Chromatography (IMAC). MBP slows down the folding of newly synthesized proteins and it directs the protein to the periplasm. This protein is used to control protein folding of complex proteins with disulfide bonds. The TEV protease recognition site allows the cleavage of this part from a fusion protein after expression.

Usage and Biology

This part can be useful to solve protein expression and purification issues of complex proteins. This part can be incorporated in front of the protein to be expressed. Afterwards, the His-tag and MBP can be cleaved.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 423
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1246
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 121

@@ Line 2: / Line 2: @@
 ===HisTag-MBP-TEVsite===
-This basic part includes a His-tag, maltose-binding protein (MBP) and a TEV protease recognition site. The His-tag can be used for protein purification with nickel affinity chromatography. MBP slows down the folding of newly synthesized proteins and it directs the protein to the periplasm. This protein is used to control protein folding of complex proteins with disulfide bonds. The TEV protease recognition site allows the cleavage of this part from a fusion protein after expression.
+This basic part includes a His-tag, maltose-binding protein (MBP) and a TEV protease recognition site. The His-tag can be used for protein purification with Immobilized Metal Affinity Chromatography (IMAC). MBP slows down the folding of newly synthesized proteins and it directs the protein to the periplasm. This protein is used to control protein folding of complex proteins with disulfide bonds. The TEV protease recognition site allows the cleavage of this part from a fusion protein after expression.
 ===Usage and Biology===
-This part can be very useful to solve protein expression and purification issues of complex proteins. This part can be incorporated in front of the protein you want to express.  Afterwards, the His-tag and MBP can be cleaved.
+This part can be useful to solve protein expression and purification issues of complex proteins. This part can be incorporated in front of the protein to be expressed.  Afterwards, the His-tag and MBP can be cleaved.
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