Difference between revisions of "Part:BBa K3183002:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3183002 short</partinfo>
 
<partinfo>BBa_K3183002 short</partinfo>
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The part was identified in the article by Lizier et al; the gene was codon optimised for <i>L. reuteri</i> and synthesized by IDT. The part was leter assembled by Gibson Assembly into the composite part <partinfo>BBa_K3183101</partinfo>, which was finally assembled into the pTRKH3 vector (<partinfo>BBa_K3183050</partinfo>), again by Gibson Assembly
  
 
<partinfo>BBa_K3183002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3183002 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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This promoter was specifically selected as an ideal promoter for <i>L. reuteri</i> because it conformed to the RF10, RF12, RF21, RF23 and RF25 standards.
 
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===Source===
 
===Source===
  
The promoter is derived from the lactate dehydrogenase (ldlL) promoter from Lactobacillus acidophilus.  
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The promoter is derived from the lactate dehydrogenase (ldlL) promoter from <i>Lactobacillus acidophilus</i>.
  
 
===References===
 
===References===
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Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.

Latest revision as of 21:35, 21 October 2019


Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp.

The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into the composite part BBa_K3183101, which was finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 219


Design Notes

This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23 and RF25 standards.

Source

The promoter is derived from the lactate dehydrogenase (ldlL) promoter from Lactobacillus acidophilus.

References

Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.