Difference between revisions of "Part:BBa K2938001:Design"

 
(→‎References)
 
Line 17: Line 17:
  
 
===References===
 
===References===
 +
[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.
 +
 +
[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.
 +
 +
[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.

Latest revision as of 13:20, 4 October 2019


Cry11Aa


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1695
    Illegal SpeI site found at 807
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1695
    Illegal SpeI site found at 807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1695
    Illegal SpeI site found at 807
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1695
    Illegal SpeI site found at 807
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Isolated from pVE4-ADRC plasmid by PCR. Optimized for E.Coli

References

[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.

[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.

[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.