Difference between revisions of "Part:BBa K2912000:Design"
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===Design Notes===
 
===Design Notes===
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The first ORF, designated rebA, extends from base 110 through 454. rebA is preceded by the potential ribosomebinding site (RBS)AGGAG, located 6 bp before the initiation codon. In E. coli, the consensus promoter sequences for the -10 and -35 regions are TTATA and TTGACA, respectively.The rebA gene uses the TAA stop codon. Termination appears to be rho dependent since there are no strong inverted repeats followed by runs of thymidine.  
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This is the first ORF of R-body, designated Reb A, extends from base 110 through 454. Reb A is preceded by the potential ribosome binding site (RBS) AGGAG, located 6 bp before the initiation codon. In E. coli, the consensus promoter sequences for the -10 and -35 regions are TTATA and TTGACA, respectively. The Reb A gene uses the TAA stop codon. Termination appears to be rho dependent since there are no strong inverted repeats followed by runs of thymidine.
  
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===Source===
  
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This part called rebA, belonging to the complete cds for the R body locus (reb) from Caedibacter taeniospiralis. The sequence is from the Genebank, and the protein ID is AAA73408.1.
  
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===Source===
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===References===
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[1] Pond FR, Gibson I, Lalucat J, et al. R-body-producing Bacteria.[J]. Microbiol Rev, 1989, 53(1): 25-67.
  
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This part called rebA, belonging to the complete cds for the R body locus (reb) from Caedibacter taeniospiralis. The source of the part will be its sequence which was retrieved from GenBank.
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[2] Matsuoka JI, Ishizuna F, Kurumisawa K, et al. Stringent Expression Control of Pathogenic R-body Production in Legume Symbiontazorhizobium Caulinodans[J]. Mbio, 2017, 8(4): 0-17.
  
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[3] Heruth DP, Pond FR, Dilts JA, et al. Characterization of Genetic Determinants for R Body Synthesis and Assembly in Caedibacter Taeniospiralis 47 and 116.[J]. Journal of Bacteriology, 1994, 176(12): 3559-3567.
  
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===References===
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[4] Quackenbush RL, Burbach JA. Cloning and Expression of Dna Sequences Associated with the Killer Trait of Paramecium Tetraurelia Stock 47[J]. Proceedings of the National Academy of Sciences of the United States of America, 1983, 80(1): 250-254.

Latest revision as of 16:23, 23 September 2019


RebA may act as a scaffolding protein to facilitate the major polymerization process


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the first ORF of R-body, designated Reb A, extends from base 110 through 454. Reb A is preceded by the potential ribosome binding site (RBS) AGGAG, located 6 bp before the initiation codon. In E. coli, the consensus promoter sequences for the -10 and -35 regions are TTATA and TTGACA, respectively. The Reb A gene uses the TAA stop codon. Termination appears to be rho dependent since there are no strong inverted repeats followed by runs of thymidine.

Source

This part called rebA, belonging to the complete cds for the R body locus (reb) from Caedibacter taeniospiralis. The sequence is from the Genebank, and the protein ID is AAA73408.1.

References

[1] Pond FR, Gibson I, Lalucat J, et al. R-body-producing Bacteria.[J]. Microbiol Rev, 1989, 53(1): 25-67.

[2] Matsuoka JI, Ishizuna F, Kurumisawa K, et al. Stringent Expression Control of Pathogenic R-body Production in Legume Symbiontazorhizobium Caulinodans[J]. Mbio, 2017, 8(4): 0-17.

[3] Heruth DP, Pond FR, Dilts JA, et al. Characterization of Genetic Determinants for R Body Synthesis and Assembly in Caedibacter Taeniospiralis 47 and 116.[J]. Journal of Bacteriology, 1994, 176(12): 3559-3567.

[4] Quackenbush RL, Burbach JA. Cloning and Expression of Dna Sequences Associated with the Killer Trait of Paramecium Tetraurelia Stock 47[J]. Proceedings of the National Academy of Sciences of the United States of America, 1983, 80(1): 250-254.